| 产品编号 | bs-5854R |
| 英文名称 | ADAM28 Rabbit pAb |
| 中文名称 | 去整合素样金属蛋白酶28抗体 |
| 别 名 | ADAM 28; MDC-L; MDCL; eMDC II; eMDCII; ADA28_HUMAN; ADAM28; Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II (eMDC II); Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L (MDC-L); 3.4.24.-; ADAM23; |
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Specific References (1) | bs-5854R has been referenced in 1 publications.
[IF=4.5] Zhang, Yu, et al. "CD19 regulates ADAM28‐mediated Notch2 cleavage to control the differentiation of marginal zone precursors to MZ B cells." Journal of Cellular and Molecular Medicine (2017). FCM ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat) |
| 产品应用 | Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 87 kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM28: 321-420/775 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ADAM28 was first described in lymphocytes, also in Thymic Endothelial Cells (TECADAM), as well as in the epididymus. Later works describe two forms of ADAM28: ADAM28-S and ADAM28-L (short and long forms). The short form is a soluble, alternatively spliced form lacking the transmembrane and cytoplasmic domains. Message levels of the short form were found to be much lower than the long form in the tissues studied, but these may be tissue-specific differences. In humans, ADAM28 is reported in lymphocytes by some authors and to be absent from mature lymphocytes by others. The thyroid, lung, trachea, esophagus, and stomach have been shown to express human ADAM28 message. Knockout mice lacking B cells and T cells express ADAM28 message in the thyroid, lung, trachea, esophagus, and stomach. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the functions of ADAM28 are still poorly understood. ADAM28 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM28 (ADAM28 Long) sequence codes for a 775 amino acid protein with a predicted mass is 87.2 kDa, and a pI of 6.50. The soluble ADAM28 (ADAM-28 Short) is a 540 amino acid sequence, with a predicted mass of 61.3 kDa, and a pI of 6.58. Other forms of ADAM28 have been reported with slightly different carboxyterminal regions.
ADAM28 may play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. It may be involved in sperm maturation. There are 2 isoforms of ADAM28: one short secreted and one long membrane bound. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. SWISS: Q9UKQ2 Gene ID: 10863 Database links: Entrez Gene: 10863 Human Omim: 606188 Human SwissProt: Q9UKQ2 Human |
| 产品图片 |
Blank control (blue line): mouse blood (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-ADAM28 antibody (bs-5854R), Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
Blank control (blue line): mouse stomach (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-ADAM28 antibody (bs-5854R),Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
