| 产品编号 | bs-5832R |
| 英文名称 | ASAP3 Rabbit pAb |
| 中文名称 | GTP酶激活蛋白ASAP3抗体 |
| 别 名 | ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 1; ACAP4; Arf GAP with SH3 domain; Arf GAP with SH3 domain, ANK repeat and PH domain containing protein 3; ARF6 GTPase activating protein; ArfGAP with SH3 domain, ankyrin repeat and PH domain; Centaurin, beta 6; CENTB6; DDEFL1; Development and differentiation enhancing factor like 1; Up regulated in liver cancer 1(UPLC1); UPLC1. |
| 研究领域 | 肿瘤 免疫学 信号转导 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 99 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ASAP3: 251-350/903 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ASAP3 is a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Function: Promotes cell proliferation. Subunit: Cytoplasm. Tissue Specificity: Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes. Similarity: Contains 2 ANK repeats. Contains 1 Arf-GAP domain. Contains 1 PH domain. SWISS: Q8TDY4 Gene ID: 55616 Database links: Entrez Gene: 55616 Human SwissProt: Q8TDY4 Human Unigene: 437379 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-ASAP3 (bs-5832R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 105 kDa
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- ASAP3 (bs-5832R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 100 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (bs-5832R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (bs-5832R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
