| 产品编号 | bs-5812R |
| 英文名称 | Rabbit Anti-PHLP antibody |
| 中文名称 | 光导蛋白样蛋白PDCL抗体 |
| 别 名 | PDCL; Phosducin Like; PhLP; Phosducin-Like Protein; PHLOP1; Phosducin like protein; PhLP1; PHLP_HUMAN. |
| 研究领域 | 细胞生物 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Chicken,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PHLP: 51-150/301 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008] Function: Acts as a positive regulator of hedgehog signaling and regulates ciliary function. Isoform 1: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers. Isoform 2: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin. Subunit: Forms a complex with the beta and gamma subunits of the GTP-binding protein, transducin. Interacts with the CCT chaperonin complex. Tissue Specificity: Expressed in buccal mucosa cell and 236 other tissues Similarity: Belongs to the phosducin family. SWISS: Q13371 Gene ID: 5082 Database links: Entrez Gene: 5082 Human Entrez Gene: 67466 Mouse Omim: 604421 Human SwissProt: Q13371 Human SwissProt: Q9DBX2 Mouse Unigene: 271749 Human Unigene: 620774 Human Unigene: 30709 Mouse Unigene: 51153 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Rat Kidney tissue lysates
Lane 7: Human HUVEC cell lysates
Lane 8: Human K562 cell lysates
Primary: Anti-PHLP (bs-5812R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PHLP) Polyclonal Antibody, Unconjugated (bs-5812R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
