| 产品编号 | bs-5807R |
| 英文名称 | Rabbit Anti-ELMO1 antibody |
| 中文名称 | 吞噬细胞运动蛋白1抗体 |
| 别 名 | CED 12; Ced 12 homolog 1; Ced 12 homolog; CED12; Ced12 homolog 1; Ced12 homolog; ELMO 1; Engulfment and cell motility 1; Engulfment and cell motility protein 1; KIAA0281; ELMO1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Cow,Chicken,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 84kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ELMO.: 581-680/727 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ELMO1 is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. ELMO1 acts in association with DOCK1 and CRK and was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. It is belived to enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Function: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Subunit: Interacts with BAI1 (By similarity). Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. Part of a complex with DOCK1 and RAC1. Part of a complex with DOCK1 and CRK isoform CRK-II. Interacts with PLEKHG6. Interacts with HCK (via SH3 domain). Subcellular Location: Cytoplasm. Cell membrane. Note=Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. Tissue Specificity: Widely expressed, with a higher expression in the spleen and placenta. Post-translational modifications: Phosphorylated by HCK (Probable). Similarity: Contains 1 ELMO domain. Contains 1 PH domain. SWISS: Q92556 Gene ID: 9844 Database links: Entrez Gene: 9844 Human Entrez Gene: 140580 Mouse Omim: 606420 Human SwissProt: Q92556 Human SwissProt: Q8BPU7 Mouse Unigene: 656638 Human Unigene: 342392 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
