| 产品编号 | bs-5786R |
| 英文名称 | KLST/Kallistatin Rabbit pAb |
| 中文名称 | 激肽释放酶抑制剂抗体 |
| 别 名 | Serpin peptidase inhibitor clade A member 4; Kallikrein binding protein; Kallikrein inhibitor; Protease inhibitor 4; Serpin A4; KAL; Kallikrein binding protein; Kallikrein inhibitor; KBP; KLST; KST; MGC108582; PI4; Protease inhibitor 4; Serpin A4; Serpin peptidase inhibitor clade A member 4; SERPINA4; KAIN_HUMAN. |
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Specific References (1) | bs-5786R has been referenced in 1 publications.
[IF=2.2] Biswas et al. Polymorphisms at the F12 and KLKB1 loci have significant trait association with activation of the renin-angiotensin system. (2016) BMC.Med.Genet. 17:21 IF ; Mouse.
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| 研究领域 | 心血管 细胞生物 免疫学 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLST/Kallistatin: 151-250/427 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Kallistatin is a serine proteinase inhibitor which binds to tissue kallikrein and inhibits its amidolytic and kininogenase activity. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity. Function: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Subunit: Monomer and some homodimers. Subcellular Location: Secreted. Tissue Specificity: Expressed by the liver and secreted in plasma. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the serpin family. SWISS: P29622 Gene ID: 5267 Database links: Entrez Gene: 5267 Human Omim: 147935 Human SwissProt: P29622 Human Unigene: 719893 Human |
| 产品图片 |
Sample:
Lane 14: Pancreas (Mouse) Lysate at 40 ug
Lane 14: Kidney (Mouse) Lysate at 40 ug
Lane 14: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-KLST/Kallistatin (bs-5786R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-60 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLST) Polyclonal Antibody, Unconjugated (bs-5786R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
