| 产品编号 | bs-5767R |
| 英文名称 | Epigen Rabbit pAb |
| 中文名称 | 上皮细胞有丝分裂蛋白抗体 |
| 别 名 | EPG; Epigen precursor; Epithelial mitogen; EPGN_HUMAN. |
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Specific References (2) | bs-5767R has been referenced in 2 publications.
[IF=38.637] Emma D. Wrenn. et al. Regulation of Collective Metastasis by Nanolumenal Signaling. Cell. 2020 Oct;183:395 IF ; Human.
[IF=4.994] Ma S et al. ErbB3 Ligand Heregulin1 Is a Major Mitogenic Factor for Uncontrolled Lung Cancer Cell Proliferation.Neoplasia. 2019 Apr;21(4):343-352. IHC-P ; Mouse.
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| 研究领域 | 肿瘤 免疫学 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Epigen: 51-130/154 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, heart and in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Function: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. Subcellular Location: Isoform 1: Membrane; Single-pass type I membrane protein (Probable). Isoform 2: Membrane; Single-pass type I membrane protein (Probable). Isoform 3: Secreted (Probable). Isoform 4: Secreted (Probable). Isoform 5: Secreted (Probable). Isoform 6: Secreted (Probable). Similarity: Contains 1 EGF-like domain. SWISS: Q6UW88 Gene ID: 255324 Database links: Entrez Gene: 255324 Human Entrez Gene: 71920 Mouse SwissProt: Q6UW88 Human SwissProt: Q924X1 Mouse Unigene: 401237 Human Unigene: 197319 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
