| 产品编号 | bs-5760R |
| 英文名称 | PTPN13 Rabbit pAb |
| 中文名称 | Fas相关磷酸酶1抗体 |
| 别 名 | APO1/CD95(Fas) associated phosphatase; Fas associated phosphatase 1; Protein tyrosine phosphatase non receptor type 13; PTP BAS; PTP BL; DKFZp686J1497; FAP 1; FAP-1; FAP1; Fas associated protein tyrosine phosphatase 1; Fas-associated protein-tyrosine phosphatase 1; HGNC:9646; hPTPE1; PNP 1; PNP1; Protein tyrosine phosphatase 1 Fas associated; protein tyrosine phosphatase 1E; Protein tyrosine phosphatase nonreceptor type 13; Protein tyrosine phosphatase PTPL1; Protein-tyrosine phosphatase 1E; Protein-tyrosine phosphatase PTPL1; PTP E1; PTP-E1; PTP1E; PTPL 1; PTPL1; PTPLE; PTPN 13; FAP1; Tyrosine protein phosphatase non receptor type 13. |
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Specific References (1) | bs-5760R has been referenced in 1 publications.
[IF=7.517] Chia-Hao Huang. et al. Cell marathon: long-distance cell migration and metastasis-associated gene analysis using a folding paper system. LAB CHIP. 2022 Sep;: ICC ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞周期蛋白 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | Flow-Cyt=3ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 273 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FAP1/PTPN13: 801-900/2485 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PTPN13 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. This PTP was found to interact with, and dephosphorylate Fas receptor, as well as I-kappa-B-alpha through the PDZ domains, which suggested its role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathway. Subunit: Interacts with TRIP6 and TNFRSF6 (Fas receptor) through its second PDZ domain. Interacts with the C-terminal SVP motif of NGFR through its third PDZ domain. Interacts with the LIM domain of PDLIM4 through its second and fourth PDZ domains. Binds PLEKHA1 and PLEKHA2 through its first PDZ domain. Interacts with BRD7 and ARHGAP29. Interacts (via PDZ 3 domain) with PKN2 (via C-terminus). Subcellular Location: Cytoplasm, cytoskeleton. Nucleus. Cell projection, lamellipodium. Tissue Specificity: Present in most tissues with the exception of the liver and skeletal muscle. Most abundant in lung, kidney and fetal brain. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 FERM domain. Contains 1 KIND domain. Contains 5 PDZ (DHR) domains. Contains 1 tyrosine-protein phosphatase domain. SWISS: Q12923 Gene ID: 5783 Database links: Entrez Gene: 5783 Human Omim: 600267 Human SwissProt: Q12923 Human Unigene: 436142 Human |
| 产品图片 |
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-FAP1 antibody (bs-5760R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
