产品编号 | bs-5753R |
英文名称 | Rabbit Anti-phospho-MLF1 Interacting Protein (Thr78) antibody |
中文名称 | 磷酸化卡波西氏肉瘤疱疹病毒潜伏核抗原相互作用蛋白1抗体 |
别 名 | MLF1 Interacting Protein/PBIP1(phospho T78); MLF1 Interacting Protein/PBIP1 (phospho T78);MLF1 Interacting Protein (phospho T78); MLF1 Interacting Protein (phospho Thr78); p-MLF1 Interacting Protein (Thr78); CENP U; CENP50; CENPU; centromere protein U; HHV8 LNAIP1; ICEN24; Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1; KLIP-1; KLIP1; KSHV latent nuclear antigen interacting protein 1; KSHV LNAIP1; LNAIP 1; LNAIP-1; MLF1IP1; PBIP1; CENPU_HUMAN. |
产品类型 | 磷酸化抗体 |
研究领域 | 肿瘤 免疫学 信号转导 细胞周期蛋白 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 48kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human MLF1 Interacting Protein around the phosphorylation site of Thr78: HS(p-T)AI |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
MLF1 Interacting protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a component of the CENPA nucleosome-associated complex (CENPA NAC), which plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. This protein is involved in transcriptional repression and interacts with the N-terminal domain of Kaposi Sarcoma Herpesvirus latent nuclear antigen (KSHV LNA). Function: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. Subunit: Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with the N-terminal domain of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen (LNA). Interacts with MLF1. Interacts with PLK1. Subcellular Location: Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates. Tissue Specificity: Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines. Post-translational modifications: Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1. SWISS: Q71F23 Gene ID: 79682 Database links: Entrez Gene: 79682 Human Entrez Gene: 71876 Mouse Omim: 611511 Human SwissProt: Q71F23 Human SwissProt: Q8C4M7 Mouse Unigene: 575032 Human Unigene: 217385 Mouse Unigene: 22108 Mouse Unigene: 128609 Rat |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |