| 产品编号 | bs-5743R |
| 英文名称 | CDKN3 Rabbit pAb |
| 中文名称 | 细胞周期蛋白依赖性激酶抑制蛋白3抗体 |
| 别 名 | Cdk associated protein phosphatase; CDI1; CDK2 associated dual specificity phosphatase; CIP2; Cyclin dependent kinase inhibitor 3; Cyclin dependent kinase interactor 1; Cyclin dependent kinase-interacting protein 2; KAP1; Kinase associated phosphatase. |
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Specific References (1) | bs-5743R has been referenced in 1 publications.
[IF=12.822] Sha Li. et al. Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma. J ADV RES. 2023 Jan;: IHC ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 细胞周期蛋白 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 24 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CDKN3: 51-150/212 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CDKN3 is a second dual specificity phosphatase that interacts with cyclin dependent kinases.The ability of CDKN3 to bind multiple cyclin-dependent kinases suggests that it may be a critical control element in cell cycle regulation, presumably by regulating the phosphorylation state of a Cdk or Cdk-associated protein. Function: May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner. Subunit: Interacts with cyclin-dependent kinases such as CDK1, CDK2 and CDK3. Does not interact with CDK4. Interacts (via C-terminus) with phosphorylated CDK2 (via C-terminal helix). Interacts with MS4A3 (via C-terminus); the interaction enhances CDKN3 enzymatic activity. Subcellular Location: Cytoplasm, perinuclear region. DISEASE: Defects in CDKN3 are found in patients with hepatocellular carcinoma (HCC) [MIM:114550]. Similarity: Belongs to the protein-tyrosine phosphatase family. SWISS: Q16667 Gene ID: 1033 Database links: Entrez Gene: 1033 Human Entrez Gene: 72391 Mouse Omim: 123832 Human SwissProt: Q16667 Human SwissProt: Q810P3 Mouse Unigene: 84113 Human Unigene: 272394 Mouse Unigene: 107220 Rat |
| 产品图片 |
2683号盒-20230324-WDD_25.9x-4-C.jpg)
Paraformaldehyde-fixed, paraffin embedded (human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN3) Polyclonal Antibody, Unconjugated (bs-5743R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
2683号盒-20230324-WDD_20.0x-1-C.jpg)
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN3) Polyclonal Antibody, Unconjugated (bs-5743R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
2683号盒-20230324-WDD_20.0x-1-C.jpg)
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN3) Polyclonal Antibody, Unconjugated (bs-5743R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
