| 产品编号 | bs-5736R |
| 英文名称 | Rabbit Anti-TACC2 antibody |
| 中文名称 | 转化酸性卷曲含蛋白质2 |
| 别 名 | TACC2_HUMAN; Anti zuai 1; AZU 1; AZU1; ECTACC; TACC 2; Transforming acidic coiled coil containing protein 2. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞周期蛋白 细胞分化 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Cow,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 309kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TACC2: 2851-2948/2948 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TACC2 is a conserved family of centrosome and microtubule interacting proteins that are implicated in cancer. This gene encodes a protein that concentrates at centrosomes throughout the cell cycle. This gene lies within a chromosomal region associated with tumorigenesis. Expression of this gene is thought to affect the progression of breast tumors. Expression of this gene is also induced by erythropoietin. Function: Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). May play a role in organizing centrosomal microtubules. May act as a tumor suppressor protein. May represent a tumor progression marker. Subunit: Interacts with CCDC100/CEP120 (By similarity). Interacts with microtubules. Interacts with YEATS4, GCN5L2 and PCAF. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. Tissue Specificity: Strongly expressed in heart, skeletal muscle, brain, prostate, thyroid and trachea. Post-translational modifications: Phosphorylated by TTK; which is required for localization in centrosome. Similarity: Belongs to the TACC family. Contains 1 SPAZ (Ser/Pro-rich AZU-1) domain. SWISS: O95359 Gene ID: 10579 Database links: Entrez Gene: 10579 Human Entrez Gene: 57752 Mouse Omim: 605302 Human SwissProt: O95359 Human SwissProt: Q811U0 Mouse Unigene: 501252 Human Unigene: 86322 Mouse Unigene: 13989 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TACC2) Polyclonal Antibody, Unconjugated (bs-5736R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
