客服热线:400-901-9800          客服QQ:4009019800          技术答疑     技术支持     质量反馈     联系我们
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-ICAM1 antibody (bs-4618R)
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:support@bioss.com.cn
说明书: 100ul  200ul
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-4618R
英文名称 ICAM1
中文名称 细胞间粘附分子-1抗体
别    名 BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor..  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  免疫学  信号转导  细胞膜受体  细胞粘附分子  细胞表面分子  糖蛋白  淋巴细胞  t-淋巴细胞  b-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 56kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD54/ICAM-1:51-100/532 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].

Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Subunit:
Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Post-translational modifications:
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
ontains 5 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P05362

Gene ID:
3383

Database links:

Entrez Gene: 3383 Human

Entrez Gene: 15894 Mouse

Entrez Gene: 25464 Rat

Omim: 147840 Human

Unigene: 643447 Human

SwissProt: P05362 Human

SwissProt: P13597 Mouse

SwissProt: Q00238 Rat

Unigene: 435508 Mouse

Unigene: 12 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample: U937 Cell (Human) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-4618R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 60 kD
Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti- ICAM1 (bs-4618R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample: Raji Cell (Human) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-4618R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1) Polyclonal Antibody, Unconjugated (bs-4618R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): U937 (fixed with 2% paraformaldehyde for 10 min at room temperature).
Primary Antibody (green line): Rabbit Anti-ICAM1 antibody (bs-4618R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
版权所有 2016-2020 www.bioss.com.cn 北京博奥森生物技术有限公司 京ICP备05066980号 京公网安备110107000727号
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100