| 产品编号 | bs-4615R |
| 英文名称 | ICAM1 |
| 中文名称 | 细胞间粘附分子1（CD54）抗体 |
| 别 名 | BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor. |
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Specific References (5) | bs-4615R has been referenced in 5 publications.
[IF=10.334] Xin-Sen Chen. et al. Losartan attenuates sepsis-induced cardiomyopathy by regulating macrophage polarization via TLR4-mediated NF-κB and MAPK signaling. PHARMACOL RES. 2022 Nov;185:106473 WB ; Mouse.
[IF=4.932] Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096 WB ; Human.
[IF=3.269] Leiming Sun. et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered. 2021;12(2):11188-11200 WB ; Rat.
[IF=2.766] Xie et al. Evaluation of Liver Ischemia-Reperfusion Injury in Rabbits Using a Nanoscale Ultrasound Contrast Agent Targeting ICAM-1. (2016) PLoS.One. 11:e0153805 IF ; Human.
[IF=1.663] Hou J et al. The expression of macrophage migration inhibitory factor and intercellular adhesion molecule-1 in rats with periodontitis and atherosclerosis. Arch Oral Biol. 2019 Aug 10;107:104513. IHC-P ; Rat.
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| 研究领域 | 肿瘤 免疫学 信号转导 干细胞 细胞粘附分子 细胞表面分子 糖蛋白 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rabbit) |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD54/ICAM-1: 451-505/532 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]. Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. Subunit: Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. Similarity: Belongs to the immunoglobulin superfamily. ICAM family. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P05362 Gene ID: 3383 Database links: Entrez Gene: 3383 Human Entrez Gene: 15894 Mouse Omim: 147840 Human Unigene: 643447 Human SwissProt: P05362 Human SwissProt: P13597 Mouse Unigene: 435508 Mouse Unigene: 12 Rat |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 30 ug Raji(Human) Cell Lysate at 30 ug Primary: Anti-ICAM1 (bs-4615R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 61 kD 
Sample:
Lung (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Lymph node (Mouse) Lysate at 40 ug Heart (Mouse) Lysate at 40 ug A549(Human) Cell Lysate at 40 ug A431(Human) Cell Lysate at 40 ug U937(Human) Cell Lysate at 40 ug K562(Human) Cell Lysate at 40 ug Primary: Anti- ICAM1 (bs-4615R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD 
Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: Huvec (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: Siha (Human) Cell Lysate at 30 ug Lane 5: U937 (Human) Cell Lysate at 30 ug Primary: Anti-ICAM1 (bs-4615R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58/100 kD Observed band size: 58 kD 
Sample:
Spleen (Mouse) Lysate at 40 ug Primary: Anti-ICAM1 (bs-4615R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD 
Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-ICAM1 antibody(bs-4615R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
