| 产品编号 | bs-5720R |
| 英文名称 | GDF10 Rabbit pAb |
| 中文名称 | 生长分化因子10抗体 |
| 别 名 | BIP; BMP 3b; BMP3B; Bone inducing protein; Bone morphogenetic protein 3b; GDF 10; GDF-10; growth differentiation factor 10; BMP3B_HUMAN; Bone-inducing protein; Growth/differentiation factor 10. |
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Specific References (1) | bs-5720R has been referenced in 1 publications.
[IF=15.266] Armaka, Marietta. et al. Single-cell multimodal analysis identifies common regulatory programs in synovial fibroblasts of rheumatoid arthritis patients and modeled TNF-driven arthritis. GENOME MED. 2022 Dec;14(1):1-25 IF ; Mouse.
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| 研究领域 | 肿瘤 免疫学 信号转导 干细胞 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12/53 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDF10: 371-478/478 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GDF10, or growth differentiation factor 10, bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. GDF10 is expressed in femur, brain, lung, skeletal muscle, pancreas and testis. Subcellular Location: Secreted. Tissue Specificity: Expressed in femur, brain, lung, skeletal muscle, pancreas and testis. Similarity: Belongs to the TGF-beta family. SWISS: P55107 Gene ID: 2662 Database links: Entrez Gene: 2662 Human Entrez Gene: 14560 Mouse GenBank: NM_004962 Human Omim: 601361 Human SwissProt: P55107 Human SwissProt: P97737 Mouse Unigene: 2171 Human Unigene: 432071 Mouse |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Pancreas tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-GDF10 (bs-5720R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band siz
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
