| 产品编号 | bs-5714R |
| 英文名称 | OVCA1 Rabbit pAb |
| 中文名称 | 卵巢癌相关基因1抗体 |
| 别 名 | Candidate tumor suppressor in ovarian cancer 1; Diphthamide biosynthesis protein 1; Diphthamide biosynthesis protein 2 homolog like 1; Diphthamide biosynthesis protein 2 like; Diptheria toxin resistance protein required for diphthamide biosynthesis(Saccharomyces) like 1; Diptheria toxin resistance protein required for diphthamide biosynthesis like 1; DPH like 1(S. cerevisiae); DPH like 1; DPH1; DPH1 homolog(S. cerevisiae); DPH1 homolog; DPH2 like 1; DPH2L; DPH2L1; HsDph1; Ovarian cancer associated gene 1; Ovarian cancer associated gene 1 protein; Ovarian tumor suppressor candidate 1; DPH1_HUMAN. |
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Specific References (1) | bs-5714R has been referenced in 1 publications.
[IF=1.871] Lin Y et al. The tumor suppressor OVCA1 is a short half‑life protein degraded by the ubiquitin‑proteasome pathway. Oncol Lett. 2019 Feb;17(2):2328-2334. WB ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OVCA1: 251-350/443 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
OVCA1 is required for the first step in the synthesis of diphthamide, a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. OVCA1 is one of several enzymes involved in synthesis of diphthamide in EEF2. Function: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity). Subunit: Interacts with DPH2 (By similarity). Interacts with RBM8A. Subcellular Location: Nucleus. Cytoplasm. Note=Punctate, primarily perinuclear localization. Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors. Similarity: Belongs to the DPH1/DPH2 family. DPH1 subfamily. SWISS: Q9BZG8 Gene ID: 1801 Database links: Entrez Gene: 1801 Human Entrez Gene: 116905 Mouse Omim: 603527 Human SwissProt: Q9BZG8 Human SwissProt: Q5NCQ5 Mouse Unigene: 513856 Human Unigene: 41496 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
