| 产品编号 | bs-4277R |
| 英文名称 | ENPP7 Rabbit pAb |
| 中文名称 | 碱性鞘磷脂酶7抗体 |
| 别 名 | ALK SMase; Alk-SMase; Alkaline sphingomyelin phosphodiesterase; E-NPP 7; Ectonucleotide pyrophosphatase/phosphodiesterase 7; Ectonucleotide pyrophosphatase/phosphodiesterase family member 7; ENPP7; ENPP7_HUMAN; Intestinal alkaline sphingomyelinase; NPP-7. |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 细胞周期蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ENPP7: 351-458/458 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Function: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Note=Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex. Tissue Specificity: Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. Post-translational modifications: N-glycosylated; required for activity and transport to the plasma membrane. Similarity: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. SWISS: Q6UWV6 Gene ID: 339221 Database links: Entrez Gene: 339221 Human Entrez Gene: 238011 Mouse SwissProt: Q6UWV6 Human Unigene: 114084 Human Unigene: 244114 Mouse Unigene: 211790 Rat |
| 产品图片 |
Sample: HT29 (human)Cell Lysate at 40 ug
Primary: Anti- ENPP7 (bs-4277R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample: FHC (human)Cell Lysate at 40 ug
Primary: Anti- ENPP7 (bs-4277R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 48 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
