| 产品编号 | bs-4276R |
| 英文名称 | ELAC2/HPC2 Rabbit pAb |
| 中文名称 | 遗传性前列腺癌蛋白2抗体 |
| 别 名 | elaC homolog 2; ElaC homolog protein 2; ELC2; Heredity prostate cancer protein 2; HPC2; putative prostate cancer susceptibility protein HPC2/ELAC2; Ribonuclease Z2; RNase Z2; tRNA 3 endonuclease 2; tRNase Z(long form); tRNase Z2; Zinc phosphodiesterase ELAC protein 2; RNZ2_HUMAN; Ribonuclease Z 2; RNase Z 2. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 92 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ELAC2: 88-190/826 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ELAC2 is a zinc phosphodiesterase which displays some tRNA 3' processing endonuclease activity. It is thought to be involved in tRNA maturation, acting by removing a 3' trailer from precursor tRNA. ELAC2 also interacts with activated Smad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expression can suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased risk of prostate cancer. A number of allelic variants have been investigated in hereditary prostate cancer. Function: Zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity. Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. Subunit: Homodimer. Interacts with PTCD1. Subcellular Location: Mitochondrion. Nucleus. Note=Mainly mitochondrial. Tissue Specificity: Widely expressed. Highly expressed in heart, placenta, liver, skeletal muscle, kidney, pancreas, testis and ovary. Weakly expressed in brain, lung, spleen, thymus, prostate, small intestine, colon and leukocytes. Similarity: Belongs to the RNase Z family. SWISS: Q9BQ52 Gene ID: 60528 Database links: Entrez Gene: 60528 Human Entrez Gene: 68626 Mouse Omim: 605367 Human SwissProt: Q9BQ52 Human SwissProt: Q80Y81 Mouse Unigene: 434232 Human Unigene: 157711 Mouse Unigene: 163124 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELAC2) Polyclonal Antibody, Unconjugated (bs-4276R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
