| 产品编号 | bs-4212R |
| 英文名称 | Rabbit Anti-Neuroligin 1 antibody |
| 中文名称 | 突触细胞粘附分子1抗体 |
| 别 名 | NLG 1; KIAA1070; MGC45115; NLG1; Neuroligin-1; NLGN1; NLGN1_HUMAN. |
| 研究领域 | 免疫学 神经生物学 细胞粘附分子 细胞表面分子 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 87kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Neuroligin 1: 701-800/863 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Neuroligin 1 is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors, channels, and signal-transduction molecules to synaptic sites of cell adhesion. In addition, the neuroligin/beta-neurexin junction may be involved in the excitatory/inhibitory specification of CNS neuron synapses. A role for Neuroligin 1 is also suggested in autism. Function: Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Subunit: Interacts with NRXN1, NRXN2 and NRXN3. Interacts with NLGN3. Interacts with AIP1 and PDZRN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3). Interacts with GOPC. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. Detected at dendritic spines. Colocalized with DLG4/PSD-95 and GRIN1/NMDAR1. Tissue Specificity: Expressed in the blood vessel walls (at protein level). Detected in brain, and at lower levels in pancreas islet beta cells. Similarity: Belongs to the type-B carboxylesterase/lipase family. SWISS: Q8N2Q7 Gene ID: 22871 Database links: Entrez Gene: 22871 Human Entrez Gene: 192167 Mouse Omim: 600568 Human SwissProt: Q8N2Q7 Human SwissProt: Q99K10 Mouse Unigene: 478289 Human Unigene: 316080 Mouse Unigene: 10173 Rat |
| 产品图片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Neuroligin 1 (bs-4212R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kD
Observed band size: 87 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-Neuroligin 1 (bs-4212R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kD
Observed band size: 87 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Neuroligin 1 (bs-4212R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kD
Observed band size: 87 kD
Blank control (blue line): U251 (fixed with 70% ethanol for 5 min at -20℃).
Primary Antibody (green line): Rabbit Anti- Neuroligin 1 antibody (bs-4212R),
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
Blank control(blue): Mouse brain cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti- Neuroligin 1 /PE Conjugated antibody (bs-4212R /PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
