| 产品编号 | bs-4205R |
| 英文名称 | hnRNP F Rabbit pAb |
| 中文名称 | 异质核糖核蛋白F抗体 |
| 别 名 | Heterogeneous nuclear ribonucleoprotein F; Heterogeneous nuclear ribonucleoprotein F; hnRNP F; HNRNPF; hnRP F; hnRPF; mcs94 1; MGC110997; Nucleolin like protein; Nucleolin like protein mcs94 1; OK/SW cl.23; Ribonucleoprotein F; HNRPF_HUMAN. |
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Specific References (1) | bs-4205R has been referenced in 1 publications.
[IF=1.664] Zhang PF et al. MicroRNA-139 suppresses hepatocellular carcinoma cell proliferation and migration by directly targeting Topoisomerase I. ONCOLOGY LETTERS 17: 1903-1913, 2019 WB ; Human.
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| 研究领域 | 细胞生物 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human hnRNP F: 301-415/415 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
hnRNP F is part of the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). These are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
hnRNP F binds to RNAs which have guanosine-rich sequences. Function: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state. Subunit: Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1. Subcellular Location: Nucleus, nucleoplasm. Tissue Specificity: Expressed ubiquitously. Post-translational modifications: Sumoylated. Similarity: Contains 3 RRM (RNA recognition motif) domains. SWISS: P52597 Gene ID: 3185 Database links: Entrez Gene: 3185 Human Entrez Gene: 98758 Mouse Omim: 601037 Human SwissProt: P52597 Human SwissProt: Q9Z2X1 Mouse Unigene: 712955 Human Unigene: 808 Human Unigene: 422979 Mouse Unigene: 476420 Mouse Unigene: 144596 Rat |
| 产品图片 |
Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated(bs-4205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated(bs-4205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
