| 产品编号 | bs-4271R |
| 英文名称 | ARHGAP20 Rabbit pAb |
| 中文名称 | Rho GTP酶激活蛋白20抗体 |
| 别 名 | Arhgap20; KIAA1391; RARHOGAP; RHG20_HUMAN; Rho GTPase activating protein 20; Rho GTPase-activating protein 20; Rho type GTPase activating protein 20; Rho-type GTPase-activating protein 20. |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞周期蛋白 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 133 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ARHGAP20: 1001-1191/1191 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Tissue Specificity: Expressed predominantly in the brain. Lower expression is found in lymph nodes. Similarity: Contains 1 PH domain. Contains 1 Ras-associating domain. Contains 1 Rho-GAP domain. SWISS: Q9P2F6 Gene ID: 57569 Database links: Entrez Gene: 57569 Human Omim: 609568 Human SwissProt: Q9P2F6 Human Unigene: 6136 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ARHGAP20) Polyclonal Antibody, Unconjugated (bs-4271R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
