产品中心-北京博奥森生物技术有限公司

产品编号	bs-4597R
英文名称	MMP-1
中文名称	基质金属蛋白酶-1抗体
别名	MMP-1; MMP1; CLGN; CLG; collagenase, fibroblast; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix metalloproteinase-1; Matrix Metalloproteinase 1; MMP1_HUMAN; OTTHUMP00000045866;MMP 1.
	Specific References (10) \| bs-4597R has been referenced in 10 publications. [IF=6.51] Woan-Ruoh Lee. et al. Laser-assisted nanoparticle delivery to promote skin absorption and penetration depth of retinoic acid with the aim for treating photoaging. INT J PHARMACEUT. 2022 Nov;627:122162 WB ; Human. PubMed:36122617 [IF=5.606] Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-3080 WB ; Human. PubMed:32417913 [IF=5.17] Ou et al. Tumor microenvironment B cells increase bladder cancer metastasis via modulation of the IL-8/androgen receptor (AR)/MMPs signals. (2015) Oncotarge. 6:26065-78 WB ; Human. PubMed:26305549 [IF=5.039] Luo P et al. IL‐37b alleviates inflammation in the temporomandibular joint cartilage via IL‐1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. WB&IHC-P ; Human&Rat. PubMed:31560411 [IF=4.162] Wenwen Chen. et al. Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Des Dev Ther. 2021; 15: 4125–4140 IHC ; mouse. PubMed:34616145 [IF=4.105] Ai Jin. et al. TSLP-induced collagen type-I synthesis through STAT3 and PRMT1 is sensitive to calcitriol in human lung fibroblasts. Bba-Mol Cell Res. 2021 Jun;:119083 IF ; Human. PubMed:34147561 [IF=3.71] Ma, Chuan, et al. (2014) "Effects of Chronic Sleep Deprivation on the Extracellular Signal-Regulated Kinase Pathway in the Temporomandibular Joint of Rats." PLoS ONE 9(9) (2014):e107544 WB ; Rat. PubMed:25226519 [IF=3.063] Li W et al. Bevacizumab tested for treatment of knee osteoarthritis via inhibition of synovial vascular hyperplasia in rabbits. Journal of Orthopaedic Translation.2019 April 28. IHC-P ; Rabbit. PubMed:doi:10.1016/j.jot.2019.04.002 [IF=2.999] Chen CL et al. miR‐134 inhibits osteosarcoma cell invasion and metastasis through targeting MMP 1 and MMP 3 in vitro and in vivo. FEBS Lett. 2019 Apr 12. WB ; Human. PubMed:30977909 [IF=2.311] Dezhi Wu. et al. Long non‑coding RNA maternally expressed gene 3 affects cell proliferation, apoptosis and migration by targeting the microRNA‑9‑5p/midkine axis and activating the phosphoinositide‑dependent kinase/AKT pathway in hepatocellular carcinoma. Oncol Lett. 2021 May;21(5):1-8 WB ; Human. PubMed:33747202
研究领域	肿瘤心血管细胞生物信号转导细胞骨架
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat,Rabbit (predicted: Dog,Cow,Horse,Sheep)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	27/41/54kDa
细胞定位	细胞外基质
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human MMP-1: 251-350/469
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Subunit: Interacts with HIV-1 Tat. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P03956 Gene ID: 4312 Database links: Entrez Gene: 4312 Human Entrez Gene: 17386 Mouse Entrez Gene: 397320 Pig Entrez Gene: 100009110 Rabbit Omim: 120353 Human SwissProt: P28053 Cow SwissProt: P03956 Human SwissProt: P33435 Mouse SwissProt: P21692 Pig SwissProt: P13943 Rabbit Unigene: 83169 Human 基质金属蛋白酶(matrix metalloproteinases, MMPs)是一族依赖锌离子而降解各种细胞外基质的蛋白酶，亦称IV型胶原酶或称明胶酶A，其主要功能为降解IV型胶原，因而它在肿瘤细胞突破基底膜屏障和浸润转移中起重要作用。目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与肿瘤转移浸润的研究。
产品图片	Sample: HL-60 Cell (Human) Lysate at 30 ug Primary: Anti-MMP-1 (bs-4597R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 55/48 kD Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-MMP-1 (bs-4597R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 55/48 kD Sample: Lane 1: Testis (Rat) Lysate at 40 ug Lane 2: Testis (Mouse) Lysate at 40 ug Primary: Anti-MMP-1 (bs-4597R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Spleen (Rabbit) Lysate at 40 ug Primary: Anti-MMP-1 (bs-4597R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 24/54 kD Observed band size: 25 kD Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (bs-4597R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记