| 产品编号 | bs-4226R |
| 英文名称 | VAPA Rabbit pAb |
| 中文名称 | 囊泡相关膜蛋白相关蛋白A抗体 |
| 别 名 | VAMP A; VAMP associated protein A; VAP 33; VAP A; VAP33; Vesicle associated membrane protein associated protein A; VAPA_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VAPA: 101-200/249 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
VAPA is a type IV membrane protein, present in the plasma membrane, intracellular vesicles and may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Two transcript variants encoding distinct isoforms have been identified. Function: May play a role in vesicle trafficking. Subunit: Homodimer, and heterodimer with VAPB. Interacts with VAMP1, VAMP2, STX1A, BET1, SEC22C and with the C-terminal domain of OCLN. Interacts with OSBPL1A. Interacts (via MSP domain) with ZFYVE27; may retain ZFYVE27 in the endoplasmic reticulum and regulate its function in cell projections formation. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Tissue Specificity: Ubiquitous. Similarity: Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. Contains 1 MSP domain. SWISS: Q9P0L0 Gene ID: 9218 Database links: Entrez Gene: 9218 Human Entrez Gene: 30960 Mouse Omim: 605703 Human SwissProt: Q9P0L0 Human SwissProt: Q9WV55 Mouse Unigene: 165195 Human Unigene: 266767 Mouse Unigene: 162275 Rat |
| 产品图片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-VAPA (bs-4226R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
