| 产品编号 | bs-4216R |
| 英文名称 | Sphingomyelin Synthase 1 Rabbit pAb |
| 中文名称 | 鞘磷脂合成酶1抗体 |
| 别 名 | MOB; Phosphatidylcholine:ceramide cholinephosphotransferase 1; Protein Mob; SGMS1; SMS 1; SMS1; TMEM23; Transmembrane protein 23; HSD36; SMS1_HUMAN; MOB. |
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Specific References (1) | bs-4216R has been referenced in 1 publications.
[IF=4.757] Tami Igarashi. et al. Horse-Derived Ceramide Accentuates Glucosylceramide Synthase and Ceramide Synthase 3 by Activating PPARβ/δ and/or PPARγ to Stimulate Ceramide Synthesis. BIOMEDICINES. 2023 Feb;11(2):548 WB ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Sphingomyelin Synthase 1: 331-413/419 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.[provided by RefSeq, Oct 2008]. Function: Sphingomyelin synthases synthesize the sphingolipid,sphingomyelin, through transfer of the phosphatidyl head group,phosphatidylcholine, on to the primary hydroxyl of ceramide. Thereaction is bidirectional depending on the respective levels of thesphingolipid and ceramide. Golgi apparatus SMS1 directly andspecifically recognizes the choline head group on the substrate,requiring two fatty chains on the choline-P donor molecule in orderto be recognized efficiently as a substrate. Major form inmacrophages. Required for cell growth in certain cell types such asHeLa cells. Suppresses BAX-mediated apoptosis and also preventscell death in response to stimuli such as hydrogen peroxide,osmotic stress, elevated temperature and exogenously suppliedsphingolipids. May protect against cell death by reversing thestress-inducible increase in levels of proapoptotic ceramide. Subcellular Location: Golgi apparatus membrane; Multi-passmembrane protein.[ALTERNATIVE PRODUCTS] Event=Alternative splicing; Namedisoforms=2; Name=1; IsoId=Q86VZ5-1; Sequence=Displayed; Name=2;IsoId=Q86VZ5-2; Sequence=VSP_027223, VSP_027224. Tissue Specificity: Brain, heart, kidney, liver, muscle andstomach. Similarity: Belongs to the sphingomyelin synthase family. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q86VZ5 Gene ID: 259230 Database links: Entrez Gene: 259230 Human Entrez Gene: 208449 Mouse Omim: 611573 Human SwissProt: Q86VZ5 Human SwissProt: Q8VCQ6 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
