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Rabbit Anti-SYT6  antibody (bs-4175R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-4175R
英文名称 SYT6
中文名称 突触蛋白6抗体
别    名 Synaptotagmin 6; Synaptotagmin VI; Synaptotagmin-6; Synaptotagmin6; SynaptotagminVI; Syt 6; Syt VI; Syt6; SYT6_HUMAN; SytVI.  
研究领域 免疫学  神经生物学  信号转导  转运蛋白  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Pig,Cow,Horse,Rabbit)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 57kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Synaptotagmin VI: 381-510/510 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene belongs to the synaptotagmin family. Synaptotagmins share a common domain structure that includes a transmembrane domain and a cytoplasmic region composed of 2 C2 domains, and are involved in calcium-dependent exocytosis of synaptic vesicles. This protein has been shown to be a key component of the secretory machinery involved in acrosomal exocytosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2011]

Function:
May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. May mediate Ca(2+)-regulation of exocytosis in acrosomal reaction in sperm (By similarity).

Subunit:
Homodimer (isoform 1). Isoform 1 forms heterodimers with SytIII, SytV and SytX. Interacts with STX1A, STX1B and STX2; the interaction is Ca(2+)-dependent. Isoform 2 is not able to form homodimer and heterodimers.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Isoform 1: Membrane; Single-pass membrane protein. Note=Localized predominantly to endoplasmic reticulum (ER) and/or Golgi-like perinuclear compartment. Isoform 2: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein.

Similarity:
Belongs to the synaptotagmin family.
Contains 2 C2 domains.

SWISS:
Q5T7P8

Gene ID:
148281

Database links:

Entrez Gene: 148281 Human

Entrez Gene: 54524 Mouse

Entrez Gene: 60565 Rat

Omim: 607718 Human

SwissProt: Q5T7P8 Human

SwissProt: Q9R0N8 Mouse

SwissProt: Q62746 Rat

Unigene: 370963 Human

Unigene: 88818 Mouse

Unigene: 48074 Rat



产品图片
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- SYT6 (bs-4175R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SynaptotagminVI Polyclonal Antibody, Unconjugated(bs-4175R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYT6) Polyclonal Antibody, Unconjugated (bs-4175R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYT6) Polyclonal Antibody, Unconjugated (bs-4175R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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