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phospho-PIK3R2 (Tyr467) Rabbit pAb (bs-5582R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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产品编号 bs-5582R
英文名称 phospho-PIK3R2 (Tyr467) Rabbit pAb
中文名称 磷酸化磷脂酰肌醇激酶p85β抗体
别    名 PIK3R2(phospho Y467); P85B_HUMAN; Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta(PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta); PI 3 Kinase p85 beta; PI 3-Kinase p85β; PI 3-Kinase p85-β; PI 3-Kinase p85 β; PI3 kinase p85 beta subunit; phosphoinositide 3 kinase regulatory subunit 2(beta); p85 beta; p85-BETA; P85B; PI3 Kinase p85 beta;  PI 3-Kinase p85β; PI 3-Kinase p85-β; PI 3-Kinase p85 β;
Specific References  (1)     |     bs-5582R has been referenced in 1 publications.
[IF=3.943] Le Tian. et al. Luteolin as an adjuvant effectively enhances CTL anti-tumor response in B16F10 mouse model. Int Immunopharmacol. 2021 May;94:107441  WB ;  Mouse.  
产品类型 磷酸化抗体 
研究领域 免疫学  信号转导  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human,Rat (predicted: Mouse,Rabbit,Cow,Dog,Horse)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 82 kDa
检测分子量
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human PIK3R2 around the phosphorylation site of Tyr467: EE(P-Y)TR 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Function:
Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

Subunit:
Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated). Interacts with NYAP1, NYAP2 and MYO16.

Post-translational modifications:
Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ.

Similarity:
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.

SWISS:
O00459

Gene ID:
5296

Database links:

Entrez Gene: 5296 Human

Omim: 603157 Human

SwissProt: O00459 Human

Unigene: 371344 Human



产品图片
Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-phospho-PIK3R2(Tyr467) (bs-5582R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 82 kD Observed band size: 82 kD
Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human A431 cell lysates Primary: Anti-phospho-PIK3R2 (Tyr467) (bs-5582R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 82 kDa Observed band size: 100 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIK3R2 (Tyr467)) Polyclonal Antibody, Unconjugated (bs-5582R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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