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phospho-PI3KCA (Tyr317) Rabbit pAb (bs-5570R)

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50ul/1180.00元

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100ul/1980.00元

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200ul/2800.00元

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产品编号	bs-5570R
英文名称	phospho-PI3KCA (Tyr317) Rabbit pAb
中文名称	磷酸化磷脂酰肌醇激酶催化亚单位A抗体
别名	PIK3CA(phospho Y317); PI3 kinase p110 subunit alpha; caPI3K; MGC142161; MGC142163; p110 alpha; Phosphatidylinositol 3 kinase catalytic alpha polypeptide; Phosphatidylinositol 3 kinase catalytic 110 KD alpha; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform; phosphoinositide 3 kinase catalytic alpha polypeptide; PI3 kinase p110 subunit alpha; PI3K; PI3KC A; PIK3C A; PIK3CA; PK3CA; PtdIns 3 kinase p110; PI3-kinase P110α; PI3-kinase p110 subunit alpha; PI 3 Kinase catalytic subunit alpha; PK3CA_HUMAN; PI3 kinase P110α.
	Specific References (28) \| bs-5570R has been referenced in 28 publications. [IF=11.161] Chi, Ming. et al. TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer. J EXP CLIN CANC RES. 2022 Dec;41(1):1-20 WB ; Human. PubMed:35581606 [IF=8.101] Zhao Yin. et al. Targeting ABCB6 with nitidine chloride inhibits PI3K/AKT signaling pathway to promote ferroptosis in multiple myeloma. FREE RADICAL BIO MED. 2023 Jul;203:86 WB ; Human,Mouse. PubMed:37044150 [IF=7.701] Liu, Zhu. et al. Comprehensive transcriptomic profiling and mutational landscape of primary gastric linitis plastica. GASTRIC CANCER. 2022 Nov;:1-17 WB ; Human. PubMed:36450891 [IF=6.575] Xin Liu. et al. Overexpression of YEATS2 Remodels the Extracellular Matrix to Promote Hepatocellular Carcinoma Progression via the PI3K/AKT Pathway. CANCERS. 2023 Jan;15(6):1850 WB ; Human. PubMed:36980736 [IF=6.376] Xu, Qinqin. et al. Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice. MOL MED. 2022 Dec;28(1):1-14 WB ; Mouse. PubMed:36463128 [IF=6.117] Minghui Zhang. et al. Selenomethionine promotes ANXA2 phosphorylation for proliferation and protein synthesis of myoblasts and skeletal muscle growth. J NUTR BIOCHEM. 2023 Feb;:109277 WB ; Mouse. PubMed:36739096 [IF=5.923] Mingfa Ling. et al. VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway. Int J Mol Sci. 2021 Jan;22(24):13352 WB ; Mouse. PubMed:34948148 [IF=5.89] Shilin Zhang. et al. CD73-Positive Small Extracellular Vesicles Derived From Umbilical Cord Mesenchymal Stem Cells Promote the Proliferation and Migration of Pediatric Urethral Smooth Muscle Cells Through Adenosine Pathway. FRONT BIOENG BIOTECH. 2022; 10: 895998 WB ; Human. PubMed:35573239 [IF=5.195] Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476 WB ; Mouse. PubMed:35724747 [IF=5.131] Kang Lu. et al. Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the α2β1/FAK/PI3K/AKT Signaling Pathway In Vitro. STEM CELLS INT. 2023 Jan 13;2023:2915826 WB ; Rat. PubMed:36684388 [IF=4.81] Yin Qin. et al. Nobiletin attenuates monocrotaline-induced pulmonary arterial hypertension through PI3K/Akt/STAT3 pathway. J PHARM PHARMACOL. 2023 May;: WB ; Rat. PubMed:37158759 [IF=4.546] Yu Fei. et al. Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer. CHIN MED-UK. 2022 Dec;17(1):1-14 WB ; Human. PubMed:36175965 [IF=4.473] Xin Jin. et al. GPRC6A is a key mediator of palmitic acid regulation of lipid synthesis in bovine mammary epithelial cells. CELL BIOL INT. 2022 Aug;: WB ; Bovine. PubMed:35979663 [IF=4.36] Kun Shi. et al. Epimedii Folium and Curculiginis Rhizoma ameliorate lipopolysaccharides-induced cognitive impairment by regulating the TREM2 signaling pathway. J Ethnopharmacol. 2022 Feb;284:114766 WB ; Mouse. PubMed:34688798 [IF=4.221] Jingtao Zhang. et al. Ze-Qi decoction inhibits non-small cell lung cancer growth and metastasis by modulating the PI3K/Akt/p53 signaling pathway. Journal of Traditional and Complementary Medicine. 2023 Mar;: WB ; Mouse. PubMed:10.1016/j.jtcme.2023.03.008 [IF=4.081] Fan Ling. et al. Antagonizing Effects of Chromium Against Iron-Decreased Glucose Uptake by Regulating ROS-Mediated PI3K/Akt/GLUT4 Signaling Pathway in C2C12. BIOL TRACE ELEM RES. 2023 May;:1-12 WB ; Mouse. PubMed:37156991 [IF=3.813] Wang P et al. MiR-29 regulates the function of Goat granulosa cell by targeting PTX3 via the PI3K/AKT/mTOR and Erk1/2 signaling pathways. J Steroid Biochem Mol Biol. 2020 Sep;202:105722. WB ; Goat. PubMed:32565247 [IF=3.571] Yu M et al. Taurine promotes milk synthesis via the GPR87-PI3K-SETD1A signaling in BMECs. J Agric Food Chem. 2019 Feb 20;67(7):1927-1936. WB ; Mouse. PubMed:30678459 [IF=3.571] Li X et al. Lysine enhances the stimulation of fatty acids on milk fat synthesis via the GPRC6A-PI3K-FABP5 signaling in bovine mammary epithelial cells. J Agric Food Chem. 2019 Jun 26;67(25):7005-7015. WB ; Cow. PubMed:31174423 [IF=3.412] Qi H et al. Methionine Promotes Milk Protein and Fat Synthesis and Cell Proliferation via the SNAT2-PI3K Signaling Pathway in Bovine Mammary Epithelial Cells.(2018) J Agric Food Chem.66(42):11027-11033. WB ; dairy cow. PubMed:30274521 [IF=3.263] Yang Yu et al. Vaccarin promotes proliferation of and milk synthesis in bovine mammary epithelial cells through the Prl receptor-PI3K signaling pathway. Eur J Pharmacol. 2020 Aug 5;880:173190. WB ; dairy cow. PubMed:32464193 [IF=2.895] Wang K et al. Neat1-miRNA204-5p-PI3K-AKT axis as a potential mechanism for Photodynamic Therapy treated Colitis in Mice. (2018) Photodiagnosis Photodyn Ther. Oct 29. WB ; Mouse. PubMed:30385297 [IF=2.813] Lu, Kang. et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling. BMC MOL CELL BIOL. 2023 Dec;24(1):1-13 WB ; Rat. PubMed:36604630 [IF=2.718] Wen Li. et al. Lysine stimulates the development of the murine mammary gland at puberty via PI3K/AKT/mTOR signalling axis. J ANIM PHYSIOL AN N. 2022 Aug;: WB ; Mouse. PubMed:35923149 [IF=2.63] Luo Yan. et al. Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis. EVID-BASED COMPL ALT. 2022;2022:4194827 WB ; Rat. PubMed:10.1155/2022/4194827 [IF=2.563] Jiang S et al. Hesperetin as an adjuvant augments protective antitumor immunity responses in B16F10 melanoma by stimulating cytotoxic CD8+ T cells. Scandinavian Journal of Immunology, 2020: e12867. WB ; Mouse. PubMed:doi:10.1111/sji.12867 [IF=2.13] Yusong Ge. et al. L-arginine stimulates the proliferation of mouse mammary epithelial cells and the development of mammary gland in pubertal mice by activating the GPRC6A/PI3K/AKT/mTOR signalling pathway. J ANIM PHYSIOL AN N. 2022 May 26 WB ; Mouse. PubMed:35616019 [IF=2.05] Fan QC et al. Integrin-α5 promoted the progression of Oral Squamous Cell Carcinoma and modulated PI3K/AKT Signaling Pathway.Arch Oral Biol. 2019 May;101:85-91. WB ; Human. PubMed:30909080
产品类型	磷酸化抗体
研究领域	肿瘤免疫学信号转导转录调节因子激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
克隆号
交叉反应	Human,Mouse (predicted: Rat)
产品应用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	124 kDa
检测分子量
细胞定位	细胞浆细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated Synthesised phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317: TP(p-Y)MN
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach. SWISS: P42336 Gene ID: 5290 Database links: Entrez Gene: 5290 Human Omim: 171834 Human SwissProt: P42336 Human Unigene: 553498 Human Unigene: 715194 Human Unigene: 85701 Human
产品图片	Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 3: Jurkat (Human) Cell Lysate at 30 ug Lane 4: MCF-7 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Lane 6: Siha (Human) Cell Lysate at 30 ug Primary: Anti-phospho-PI3KCA (Tyr317) (bs-5570R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/120 kD Observed band size: 110 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PI3KCA (Tyr317)) Polyclonal Antibody, Unconjugated (bs-5570R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记