| 产品编号 | bs-5564R |
| 英文名称 | phospho-PRKD3 (Ser41) Rabbit pAb |
| 中文名称 | 磷酸化蛋白激酶C nu型抗体 |
| 别 名 | PRKD3(phospho S41); PKD/PKCμ(phospho-Ser41); EPK 2; EPK2; EPK-2; nPKC nu; nPKCnu; nu; PKCnu; PKD 3; PKD3; PRK D3; PRKCN; PRKD 3; PRKD3; Protein kinase C; Protein kinase C nu; Protein kinase C nu type; Protein kinase D3; Protein kinase EPK 2; Protein kinase EPK2; Serine threonine protein kinase; Serine/threonine protein kinase D3; KPCD3_HUMAN. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 免疫学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Cow,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 100 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PRKD3 around the phosphorylation site of Ser41: RL(p-S)NG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Protein kinase C (PKC) is a family of serine and threonine specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members. Function: Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress. Subcellular Location: Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation. Tissue Specificity: Ubiquitous. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. SWISS: O94806 Gene ID: 23683 Database links: Entrez Gene: 23683 Human Entrez Gene: 75292 Mouse Omim: 607077 Human SwissProt: O94806 Human SwissProt: Q8K1Y2 Mouse Unigene: 660757 Human Unigene: 716034 Human Unigene: 252776 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-PRKD3(Ser41)) Polyclonal Antibody, Unconjugated (bs5564R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-PRKD3(Ser41)) Polyclonal Antibody, Unconjugated (bs5564R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
