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phospho-PKMYT1 (Thr495) Rabbit pAb (bs-5531R)  
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产品编号 bs-5531R
英文名称 phospho-PKMYT1 (Thr495) Rabbit pAb
中文名称 磷酸化蛋白激酶PKMYT1(Thr495)抗体
别    名 PKMYT1(phospho T495); cdc2 inhibitory kinase; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; DKFZp547K1610; FLJ20093.  
Specific References  (2)     |     bs-5531R has been referenced in 2 publications.
[IF=6.714] Xu,et al.Ablation of PPARγ in subcutaneous fat exacerbates age-associated obesity and metabolic decline.(2018) Aging Cell. 17:.  WB ;  Mouse.  
[IF=3.82] Xu,et al.Adipocytes affect castration-resistant prostate cancer cells to develop the resistance to cytotoxic action of NK cells with alterations of PD-L1/NKG2D ligand levels in tumor cells.(2018) The Prostate. 78:353-364.  WB ;  Human.  
产品类型 磷酸化抗体 
研究领域 免疫学  信号转导  细胞周期蛋白  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human
产品应用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 55 kDa
检测分子量
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human PKMYT1 around the phosphorylation site of Thr495: ED(p-T)LD 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Function:
Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

Subunit:
Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.

Subcellular Location:
Endoplasmic reticulum membrane. Golgi apparatus membrane.

Post-translational modifications:
Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain.

SWISS:
Q99640

Gene ID:
9088

Database links:

Entrez Gene: 9088 Human

Entrez Gene: 268930 Mouse

Entrez Gene: 287101 Rat

Omim: 602474 Human

SwissProt: Q99640 Human

SwissProt: Q9ESG9 Mouse

Unigene: 77783 Human

Unigene: 182193 Mouse

Unigene: 204349 Rat



产品图片
Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Lane 3: Molt-4 (Human) Cell Lysate at 30 ug Lane 4: K562 (Human) Cell Lysate at 30 ug Lane 5: U2os (Human) Cell Lysate at 30 ug Lane 6: U251 (Human) Cell Lysate at 30 ug Lane 7: A549 (Human) Cell Lysate at 30 ug Primary: Anti-phospho-PKMYT1 (Thr495) (bs-5531R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
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