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phospho-MAP4K4 (Ser801) Rabbit pAb (bs-5493R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-5493R
英文名称 phospho-MAP4K4 (Ser801) Rabbit pAb
中文名称 磷酸化丝裂原活化蛋白激酶MAP4K4抗体
别    名 MAP4K4(phospho Ser801); MAP4K4(phospho S801); Hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; KIAA0687; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK.  
产品类型 磷酸化抗体 
研究领域 肿瘤  免疫学  信号转导  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human,Rat (predicted: Mouse,Rabbit,Chicken,Dog,Horse)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 142 kDa
检测分子量
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human MAP4K4 around the phosphorylation site of Ser801: AS(p-S)LN 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 MAP4K4 (Mitogen-activated protein kinase kinase kinase kinase 4) belongs to the serine/threonine protein kinase family and specifically activates MAPK8/JNK. It may play a role in the response to environmental stress and is thought to function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF alpha signaling pathway. It interacts with the SH3 domain of the adapter protein Nck. MAP4K4-dependent signaling inhibits PPARG responsive gene expression, adipogenesis, and insulin stimulated glucose transport.

Function:
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.

Subunit:
Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially).

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.

SWISS:
O95819

Gene ID:
9448

Database links:

Entrez Gene: 9448 Human

Omim: 604666 Human

SwissProt: O95819 Human

Unigene: 431550 Human



产品图片
Sample: Hela Cell (Human) Lysate at 30 ug HepG2 Cell (Human) Lysate at 30 ug Primary: Anti- p-MAP4K4 (Ser801) (bs-5493R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 142kD Observed band size: 135kD
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phosphor-MAP4K4) Polyclonal Antibody, Unconjugated (bs-5493R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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