| 产品编号 | bs-0102M |
| 英文名称 | PKM2 |
| 中文名称 | 丙酮酸激酶-M2抗体 |
| 别 名 | PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; Tumor M2-PK; p58; OIP-3; KPYM_HUMAN. |
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Specific References (1) | bs-0102M has been referenced in 1 publications.
[IF=3.9] Xu Wei-long. et al. Quercetin Antagonizes Glucose Fluctuation Induced Renal Injury by Inhibiting Aerobic Glycolysis via HIF-1α/miR-210/ISCU/FeS Pathway. Front Med-Lausanne. 2021 Mar;8:219 WB ; Mouse.
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| 研究领域 | 肿瘤 免疫学 信号转导 细胞周期蛋白 激酶和磷酸酶 肿瘤细胞生物标志物 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse,Human (predicted: Rabbit,Horse,Cow,Pig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PKM2: 51-150/531 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Subunit: Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. Tissue Specificity: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Post-translational modifications: ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Similarity: Belongs to the pyruvate kinase family. SWISS: P14618 Gene ID: 5315 Database links: Entrez Gene: 5315 Human Entrez Gene: 18746 Mouse Omim: 179050 Human SwissProt: P14618 Human SwissProt: P52480 Mouse Unigene: 534770 Human Unigene: 326167 Mouse Unigene: 405069 Mouse Unigene: 1556 Rat 丙酮酸激酶（PK）有L、R、M1、M2四种同功酶，均为四聚体。 L型主要分布于肝脏，R型存在于红细胞，在结构、免疫学和动力学上十分相似，由同一基因调控；M1存在于肌肉中，M2分布于除上述以外的其他组织（尤以肾脏最多）以及胎儿各组织。PK也是一种癌胚蛋白，在恶性肿瘤中，增高的都是M2型。 |
| 产品图片 |
Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: U87MG (Human) Cell Lysate at 30 ug Lane 3: U251 (Human) Cell Lysate at 30 ug Primary: Anti-PKM2 (bs-0102M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD 
Sample:
Hela Cell (Human) Lysate at 40 ug NIH/3T3 Cell (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Jurkat Cell (Human) Lysate at 40 ug Primary: Anti-PKM2 (bs-0102M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD 
Sample:
MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-PKM2 (bs-0102M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD 
Tissue/cell: Human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated(bs-0102M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining 
U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (bs-0102M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (bs-0102M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: rat colitis tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated(bs-0102M) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Mouse IgG, Cy5 conjugated(bs-0296G-Cy5)used at 1:200 dilution for 40 minutes at 37°C. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
