| 产品编号 | bs-4529R |
| 英文名称 | NDV HN |
| 中文名称 | 鸡新城疫血凝素－神经氨酸酶抗体 |
| 别 名 | hemagglutinin-neuraminidase; newcastle disease virus(NDV); HN; hemagglutinin-neuraminidase protein [Newcastle disease virus]; HN_NDVB; Newcastle disease virus HN; Newcastle disease virus hemagglutinin neuraminidase. |
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Specific References (2) | bs-4529R has been referenced in 2 publications.
[IF=4.43] Wei, Ding, et al. "Oncolytic Newcastle disease virus expressing chimeric antibody enhanced anti-tumor efficacy in orthotopic hepatoma-bearing mice."Journal of Experimental & Clinical Cancer Research 34.1 (2015): 1. IHC-P ; Mouse.
[IF=3.24] Bo-Kyoung Jung. et al. The human ACE-2 receptor binding domain of SARS-CoV-2 express on the viral surface of the Newcastle disease virus as a non-replicating viral vector vaccine candidate. Plos One. 2022 Feb;17(2):e0263684 WB ; Monkey.
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| 研究领域 | 免疫学 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: NDV) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from NDV HN protein: 401-500/577/577 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The entry of Newcastle disease virus (NDV), a prototype paramyxovirus, is directed by two virion glycoproteins, the hemagglutinin-neuraminidase (HN) protein and the fusion (F) protein . HN protein, the virus attachment protein, binds to sialic acid-containing receptors, and F protein mediates membrane fusion. In contrast to many viral fusion proteins, paramyxovirus F proteins do not require the acid pH of endosomes to activate fusion activity. As a consequence, infected cells expressing both attachment proteins and F proteins can fuse with adjacent cells to form multinuclear cells, or syncytia, a process that is assumed to mimic virus-cell fusion . Function: Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity). Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins (By similarity). Subcellular Location: Virion membrane; Single-pass type II membrane protein (Potential). Host cell membrane; Single-pass type II membrane protein (Potential). Similarity: Belongs to the paramyxoviruses hemagglutinin-neuraminidase family. SWISS: P32884 Gene ID: N/A Database links: |
| 产品图片 |
Sample:
Lane 1: Recombinant NDV HN protein, His Primary: Anti-NDV HN (bs-4529R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 61 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
