| 产品编号 | bs-4608R |
| 英文名称 | CUEDC2 Rabbit pAb |
| 中文名称 | CUE结构域蛋白2抗体 |
| 别 名 | bA18I14.5; C10orf66; Chromosome 10 open reading frame 66; CUE domain containing 2; CUE domain-containing protein 2; CUED2; CUED2_HUMAN; Cuedc2; MGC2491; OTTHUMP00000020368. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 信号转导 激酶和磷酸酶 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CUE domain containing 2: 201-287/287 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Controls PGR and ESR1 protein levels through their targeting for ubiquitination and subsequent proteasomal degradation. Function: Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism. Subunit: Interacts with PGR. Interacts with ESR1 in the presence or absence of 17 beta-estradiol. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Significantly up-regulated in breast tumor tissues compared with matched adjacent normal tissues (at protein level). Levels inversely correlate with ESR1 in breast cancers and are lower in low-grade tumors compared to high-grade tumors. DISEASE: Note=May predict the clinical outcome of tamoxifen therapy of breast cancer patients. Patients with tumors that highly express CUEDC2 do not respond to tamoxifen treatment as effectively as those with tumors with low expression. Similarity: Belongs to the CUEDC2 family. Contains 1 CUE domain. SWISS: Q9H467 Gene ID: 79004 Database links: Entrez Gene: 79004 Human Entrez Gene: 67116 Mouse Omim: 614142 Human SwissProt: Q9H467 Human SwissProt: Q9CXX9 Mouse Unigene: 500874 Human Unigene: 218848 Mouse Unigene: 19673 Rat CUEDC2(CUE Domain Containing 2)能够结合IKKα和IKKβ,并通过使IKKα和IKKβ去磷酸化而抑制NF-κ信号通路。CUEDC2能特异性结合雌激素受体（ER），并导致ER的泛素化降解.抑制乳腺癌细胞的生长。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CUEDC2) Polyclonal Antibody, Unconjugated (bs-4608R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
