| 产品编号 | bs-5376R |
| 英文名称 | phospho-G3BP1 (Ser232) Rabbit pAb |
| 中文名称 | 磷酸化Ras GTP酶活化蛋白结合蛋白1抗体 |
| 别 名 | G3BP(phospho S232); p-G3BP(phospho S232); G3BP-1(phospho-Ser232); ATP dependent DNA helicase VIII; G3BP; G3BP1; GAP binding protein; GAP SH3 domain binding protein 1; HDH VIII; MGC111040; Ras GTPase activating protein binding protein 1; Ras GTPase activating protein SH3 domain binding protein; RasGAP associated endoribonuclease G3BP. |
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Specific References (1) | bs-5376R has been referenced in 1 publications.
[IF=5.06] Luo, Rui, et al. "Label-free quantitative phosphoproteomic analysis reveals differentially regulated proteins and pathway in PRRSV infected pulmonary alveolar macrophages." Journal of Proteome Research (2014). WB ; Pig.
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| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Rabbit,Pig,Cow) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52-60 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human G3BP1 around the phosphorylation site of Ser232: SS(p-S)PA |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. Function: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Subcellular Location: Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. Arg-435 is dimethylated, probably to asymmetric dimethylarginine. Similarity: Contains 1 NTF2 domain. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q13283 Gene ID: 10146 Database links: Entrez Gene: 10146 Human Entrez Gene: 27041 Mouse Omim: 608431 Human SwissProt: Q13283 Human SwissProt: P97855 Mouse Unigene: 587054 Human Unigene: 380129 Mouse Unigene: 39631 Mouse Unigene: 405739 Mouse Unigene: 25201 Rat |
| 产品图片 |
Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human HeLa cell lysates
Primary: Anti-phospho-G3BP1 (Ser232) (bs-5376R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52-60 kDa
Observed band size: 62 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
