| 产品编号 | bs-4095R |
| 英文名称 | Rabbit Anti-CTNNBIP1 antibody |
| 中文名称 | β链接素相互作用蛋白1抗体 |
| 别 名 | Beta catenin interacting protein 1; Beta catenin interacting protein ICAT; Catenin beta interacting protein 1; CTNNBIP 1; ICAT; Inhibitor of beta catenin and Tcf 4; MGC15093; CNBP1_HUMAN. |
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Specific References (1) | bs-4095R has been referenced in 1 publications.
[IF=6.52] Qi, Wei, et al. "Targeting the Wnt‐regulatory protein CTNNBIP1 by microRNA‐214 enhances the stemness and self‐renewal of cancer stem‐like cells in lung adenocarcinomas." STEM CELLS (2015). IHC-F ; Human.
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| 研究领域 | 细胞生物 免疫学 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 9kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CTNNBIP1: 3-70/81 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CTNNBIP1 (Catenin, beta interacting protein 1) binds to beta-catenin and prevents the interaction between beta-catenin and TCF transcription factor family members. It is a negative regulator of the Wnt signaling pathway. Function: Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway. Subunit: Binds CTNNB1. Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Similarity: Belongs to the CTNNBIP1 family. SWISS: Q9NSA3 Gene ID: 56998 Database links: Entrez Gene: 56998 Human Entrez Gene: 67087 Mouse Omim: 607758 Human SwissProt: Q9NSA3 Human SwissProt: Q9JJN6 Mouse Unigene: 463759 Human Unigene: 299735 Mouse Unigene: 162132 Rat |
| 产品图片 |
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- CTNNBIP1 (bs-4095R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 9 kD
Observed band size: 9 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (bs-4095R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (bs-4095R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
