产品编号 | bs-4082R |
英文名称 | Rabbit Anti-phospho-LATS2 (Ser83) antibody |
中文名称 | 磷酸化肿瘤抑制基因LATS2抗体 |
别 名 | Kinase phosphorylated during mitosis; KPM; Large tumor suppressor homolog 2; Large tumor suppressor homolog 2 Drosophila; LATS large tumor suppressor Drosophila homolog 2; LATS large tumor suppressor homolog 2; Serine/threonine kinase kpm; Serine/threonine protein kinase kpm; Warts like kinase; FLJ13161; Kinase phosphorylated during mitosis protein; Large tumor suppressor kinase 2; Lats2; LATS2_HUMAN. |
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Specific References (2) | bs-4082R has been referenced in 2 publications.
[IF=5.682] Xinhao Wang. et al. Yes-associated protein reacts differently in vascular smooth muscle cells under different intensities of mechanical stretch. Aging-Us. 2022 Jan 15; 14(1): 286–296 WB ; Rat.
[IF=3.457] Li L et al. The inhibition of Hippo/Yap signaling pathway is required for magnesium isoglycyrrhizinate to ameliorate hepatic stellate cell inflammation and activation.Biomed Pharmacother. 2018 Oct;106:83-91. ICF ; Mouse.
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产品类型 | 磷酸化抗体 |
研究领域 | 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 120-150kDa |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human LATS2 around the phosphorylation site of Ser83: RY(p-S)LL |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Subunit: Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Tissue Specificity: Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 UBA domain. SWISS: O95835 Gene ID: 26524 Database links: Entrez Gene: 26524 Human Entrez Gene: 50523 Mouse Omim: 604861 Human SwissProt: Q9NRM7 Human SwissProt: Q7TSJ6 Mouse Unigene: 78960 Human Unigene: 347899 Mouse |
产品图片 |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-LATS2 (Ser83) Polyclonal Antibody, Unconjugated(bs-4082R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |