| 产品编号 | bs-5284R |
| 英文名称 | phospho-CD244 (Tyr271) Rabbit pAb |
| 中文名称 | 磷酸化CD244抗体 |
| 别 名 | CD244(phospho Y271); CD244(phospho-Tyr271); NKR2B4; SLAMF4; natural killer cell receptor 2B4 isoform 2 precursor; 2B4; C9.1; CD244 antigen; CD244 molecule natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; F730046O15Rik; h2B4; Ly90; NAIL; Natural killer cell activation-inducing ligand; Natural killer cell receptor 2B4; NK cell activation inducing ligand; NK cell activation inducing ligand NAIL; NK cell type I receptor protein 2B4; Nmrk; Non-MHC restricted killing associated; OTTHUMP00000027884; p38; SLAM family, member 4. |
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Specific References (1) | bs-5284R has been referenced in 1 publications.
[IF=4.047] Xu Xiaoyan. et al. A novel 2B4 receptor leads to worse pregnancy outcomes by facilitating TNF-α and IFN-γ production in dNK cells during Toxoplasma gondii infection. PARASITE VECTOR. 2022 Dec;15(1):1-15 WB ; Human.
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| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 细胞凋亡 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Rabbit,Pig,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human CD244 around the phosphorylation site of Tyr271: TI(p-Y)ED |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CD244 is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. CD244 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Although there have been several molecules shown to interact with CD244, the signaling mechanism ofCD244-mediated activation of NK cells is still unknown. It is a high affinity receptor for CD48. Function: Modulate other receptor-ligand interactions to enhance leukocyte activation. Subunit: Interacts with CD48. Following phosphorylation, it is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. Binding of SH2D1A/SAP to CD244 prevents its association with PTPN11/SHP-2. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Tissue Specificity: Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. Similarity: Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: Q9BZW8 Gene ID: 51744 Database links: Entrez Gene: 51744 Human Entrez Gene: 18106 Mouse Omim: 605554 Human SwissProt: Q9BZW8 Human SwissProt: Q07763 Mouse Unigene: 157872 Human Unigene: 2299 Mouse Unigene: 113760 Rat Unigene: 53994 Rat |
| 产品图片 |
Sample: Hela (Human) Lysate at 40 ug
Primary: Anti- phospho-CD244(Tyr271) (bs-5284R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 65 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-CD244(Tyr271) (bs-5284R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 70 kD
Blank control: U937.
Primary Antibody (green line): Rabbit Anti-CD244 antibody (bs-5284R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
