| 产品编号 | bs-5148R |
| 英文名称 | Cdc7 Rabbit pAb |
| 中文名称 | 细胞分裂周期蛋白7抗体 |
| 别 名 | Cdc 7; Cdc-7; CDC7 Kinase; CDC7 cell division cycle 7; CDC7 cell division cycle 7 like 1; CDC7 L1; Cdc7 like 1; CDC7 related kinase; CDC7L 1; CDC7L1; Cell division cycle 7 homolog; Cell division cycle 7 kinase; Cell division cycle 7 like protein 1; Cell division cycle 7 related protein kinase; HsCDC 7; HsCDC7; Hsk 1; Hsk1; HuCDC 7; CDC7_HUMAN. |
| 研究领域 | 免疫学 信号转导 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cdc7: 151-250/574 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair. Function: Seems to phosphorylate critical substrates that regulatethe G1/S phase transition and/or DNA replication. Canphosphorylates MCM2 and MCM3. Subunit: Forms a complex with either DBF4/DBF4A or DBF4B, leadingto the activation of the kinase activity. Subcellular Location: Nucleus. Similarity: elongs to the protein kinase superfamily. Ser/Thrprotein kinase family. CDC7 subfamily. Contains 1 protein kinase domain. SWISS: O00311 Gene ID: 8317 Database links: Entrez Gene: 8317 Human Omim: 603311 Human SwissProt: O00311 Human Unigene: 533573 Human |
| 产品图片 |
Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cdc7 Polyclonal Antibody, Unconjugated(bs-5148R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Cdc7/FITC Conjugated antibody (bs-5148R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
