| 产品编号 | bs-0994P |
| 英文名称 | SLT/SLT-IIe (O139) protein |
| 中文名称 | 猪水肿素蛋白 |
| 别 名 | SLT/SLT-IIe(O139) immunoaffinty purified full length proten; Shiga-Like Toxin Type II Variant protein, Immuno affinity chromatography purified. |
| 理论分子量 | 35kDa |
| 性 状 | Lyophilized or Liquid |
| 物 种 | E.coli |
| 序 列 | Purified native protein |
| 内毒素 | Not analyzed |
| 活性 | Yes |
| 缓 冲 液 | 10 mM TBS (pH=7.4). |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Shiga-like toxin type II (SLT-II) and Shiga-like toxin type II variant (SLT-IIv) are cytotoxins produced by certain strains of Escherichia coli. Nucleotide sequence analyses had revealed that the structural genes for the A subunit and B subunit of SLT-II or SLT-IIv are arranged in an operon. Primer extension and S1 nuclease protection analyses identified a promoter for the slt-II operon 118 bases upstream of the slt-IIA gene. The slt-IIv promoter was demonstrated to be identical to the slt-II promoter. The slt-II and slt-IIv promoters differed significantly from the previously characterized Shiga toxin (stx) and Shiga-like toxin type 1 (slt-I) promoters. The transcriptional efficiencies of the stx and slt-II promoters were compared in fusions to the chloramphenicol acetyltransferase gene, and constitutive expression of the slt-II promoter was found to be equivalent to derepressed expression of the stx promoter. In contrast to the stx and slt-I promoters, the slt-II and slt-IIv promoters did not contain sequences for binding of the Fur repressor protein, and SLT-II production was not determined by iron levels in the media in various E. coli strains with wild-type or mutant ferric uptake regulation (fur) alleles. Northern (RNA) blot analysis demonstrated a single mRNA transcript for the slt-II operon, and further analysis of the slt-II operon by primer extension did not reveal an independent promoter for the B subunit gene. A putative rho-independent transcription terminator was identified 274 bases downstream of slt-IIB. These data indicated that the slt-II and slt-IIv operons differ from the stx/slt-I operon in regulation of their transcription by iron. Whether these regulatory differences enable the type I and type II groups of Shiga-like toxins to perform different roles in the pathogenesis of infectious diseases remains to be established. SWISS: N/A Gene ID: N/A 致病性大肠肝菌-志贺样毒素Ⅱ型变异体，又名猪水肿素（Shiga-Like Toxin Type II Variant），为病原性大肠肝菌的毒素-SLT-IIe属于类志贺氏毒素家族成员。该毒素与其家族其他成员有着较高的同源性，SLT-IIe为VTEC(或SLTEC)所产生的一种蛋白质性质的细胞毒素，是引起人和动物出血性腹泻和猪水肿病（仔猪、羊）的主要毒力因子。常见病原菌的血清型是O139。 猪水肿素主要引起猪水肿病又名猪胃肠水肿。是由病原性大肠肝菌的毒素引起断奶仔猪的一种急性散发性疾病。主要表现为突然发病，运动共济失调，惊厥，局部或全身麻痹及头部水肿。剖检变化为头部皮下，胃壁及大肠间膜的水肿。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
