| 产品编号 | bs-0362P |
| 英文名称 | Recombinant protein A |
| 中文名称 | 重组蛋白A/重组金黄色葡萄球菌蛋白A |
| 别 名 | Immunoglobulin G-binding protein A; SPA; Recombinant protein A; Staphylococcal protein A; IgG-binding protein A; SPA_STAA8; ProteinA recA蛋白; 重组蛋白-A; |
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Specific References (4) | bs-0362P has been referenced in 4 publications.
[IF=6.556] Lizhong Zhang. et al. Ultra-precise weak measurement-based interfacial biosensors. TALANTA. 2022 Dec;:124217
[IF=3.43] Qi, Honglan, Chen Wang, and Ning Cheng. "Label-free electrochemical impedance spectroscopy biosensor for the determination of human immunoglobulin G." Microchimica Acta 170.1-2 (2010): 33-38. Other ;
[IF=3.38] Ma, Qiufeng, et al. "Construction and operation of a microrobot based on magnetotactic bacteria in a microfluidic chip." Biomicrofluidics 6 (2012): 024107. other ;
[IF=3.286] Li Q et al. Oriented assembly of surface plasmon resonance biosensor through staphylococcal protein A for the chlorpyrifos detection. Anal Bioanal Chem. 2019 Jul 5. Other ; Monkey.
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| 克 隆 号 | |
| 理论分子量 | 34 kDa |
| 检测分子量 | 45-47 |
| 性 状 | Lyophilized |
| 物 种 | S.aureus |
| 序 列 | 37-469/516 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | Not analyzed |
| 表达系统 | E.coli |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. |
| 产品图片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
