| 产品编号 | bsF-P0002 |
| 英文名称 | KLH / FITC |
| 中文名称 | FITC标记血蓝蛋白 |
| 别 名 | Keyhole limpet; keyhole limpet hemocyanin; Hemocyanin from Megathura crenulata (keyhole limpet); Hemocyanin from Limulus polyphemus hemolymph; hemocyanin; haemocyanin; KLH / FITC 血蓝素; 钥孔血蓝蛋白; |
| 性 状 | Liquid |
| 浓 度 | >1.0 mg/ml |
| 物 种 | N/A |
| 序 列 | Purified native protein |
| 内毒素 | Not analyzed |
| 活性 | Not tested |
| 缓 冲 液 | 10 mM TBS (pH=7.4) with 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier proteins for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific antibodies. In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically based research techniques in mammalian model organisms. KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory. A high quality KLH preparation with the clear opalescent blue color is the best indicator of KLH solubility. 绿色荧光素（FITC）标记标记血蓝蛋白 产 品 说 明 荧光素标记抗体（FA）是目前广泛用于免疫病理、细胞化学、流氏细胞学、病理学及自身抗体的临床免疫中的特异、灵敏、定性和定位相结合的免疫化学试剂。 我公司的荧光素抗体为本公司制备的抗血清，再经过亲和层析纯化或特异性免疫亲和层析纯化的方法纯化为抗体的IgG成分，并采用直接标记法，将异硫氰酸荧光素（FITC美国sigma荧光素产品）共价交联到抗体血管紧张素转换酶（IgG）分子的赖氨酸分子上，经过标记偶联、低温透析、HPLC分离纯化、分子筛过滤、浓缩管浓缩、蛋白定量、标记后浓度鉴定、检测、分装、真空冷冻抽干而制备完成。 应用范围： 1.免疫组织及免疫病理中抗原、抗体检查，如：各肿瘤抗原检查，细胞凋亡检测。 2.用于细胞因子等检查的荧光流式细胞仪测定。 3.用于临床自身免疫病的多种自身抗体荧光检查，如：抗核抗体，抗心肌酶抗体. 4.白细胞分型抗原的免疫荧光染色。 5.用于细菌、病毒、梅毒螺旋体、钩端螺旋体等微生物的抗原及抗体检测。 主要性能： 0.5ml或根据试验需求的各种包装，并提供浓缩液或冻干粉，含有0.01%NaN3防腐剂。 血蓝蛋白含量在1mg/1ml左右.FITC为Sigma异构体I,约在70-80ug/ml,495nm为最大吸收峰，激发波长 525 nm . 工作浓度： 用0.01mol/l pH7.4 PBS 作1：20–1：50稀释使用。 保存：-20°C可保存一年，避免反复冻融。 我公司可根据您科研的需求提供各种标记服务 ，标记各种符合标记条件的蛋白及抗体，质量保证，用时短，价格合理。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
