| 产品编号 | bs-0296P-FITC |
| 英文名称 | Mouse IgG / FITC |
| 中文名称 | FITC标记小鼠IgG（同型对照) |
| 别 名 | Mouse Immunoglobulin G/FITC 荧光素FITC标记小鼠IgG(细胞流式同型对照) |
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Specific References (1) | bs-0296P-FITC has been referenced in 1 publications.
[IF=9.518] Du S et al. A portable immune-thermometer assay based on the photothermal effect of graphene oxides for the rapid detection of Salmonella typhimurium. Biosens Bioelectron. 2019 Sep 6;144:111670. Other ;
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| 性 状 | Liquid |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 产品图片 |
Blank control:Hela.
Isotype Control:Mouse IgG-FITC(bs-0296P-FITC)
Dilution: 1ug/Test;
Primary Antibody (green line): Mouse Anti-EGFR antibody (bs-33050M-FITC)
Dilution: 1ug/Test;
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Isotype control antibody (orange line) was mouse IgG. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
