产品编号 | bs-0312R |
英文名称 | Rabbit Anti-Avidin antibody |
中文名称 | 兔抗亲和素抗体 |
别 名 | AVD; LOC396260 |
Specific References (1) | bs-0312R has been referenced in 1 publications.
[IF=2.562] Xu Pengfei. et al. MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis. BMC MUSCULOSKEL DIS. 2022 Dec;23(1):1-9 WB ; Human.
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抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Species independent |
产品应用 | WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:200-1000,ELISA=1:2000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Lyophilized |
免 疫 原 | Native Avidin: full length |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M PBS (pH7.4). |
保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity. In its tetrameric form, avidin is estimated to be between 66–69 kDa in size.Ten percent of the molecular weight is attributed to carbohydrate content composed of four to five mannose and three N-acetylglucosamine residues. The carbohydrate moieties of avidin contain at least three unique oligosaccharide structural types that are similar in structure and composition.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |