| 产品编号 | bs-5137R |
| 英文名称 | TFEB |
| 中文名称 | T淋巴细胞转录调节因子TFEB抗体 |
| 别 名 | Alpha TFEB; AlphaTFEB; bHLHe35; T cell transcription factor EB; T-cell transcription factor EB; TCFEB; Transcription factor EB; TFEB_HUMAN. |
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Specific References (6) | bs-5137R has been referenced in 6 publications.
[IF=11.092] Juan Cen. et al. A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation. ADV HEALTHC MATER. 2022 Sep;:2200817 WB, IHC ; Rat, Human.
[IF=10.723] Ke Zhang. et al. Aloe gel glucomannan induced colon cancer cell death via mitochondrial damage-driven PINK1/Parkin mitophagy pathway. CARBOHYD POLYM. 2022 Nov;295:119841 IF ; Mouse.
[IF=5.988] Liu Haoge. et al. Number 2 Feibi Recipe Ameliorates Pulmonary Fibrosis by Inducing Autophagy Through the GSK-3β/mTOR Pathway. FRONT PHARMACOL. 2022 Jul;0:2714 IF ; Mouse.
[IF=5.396] Yujia Zhou. et al. Lysosome-mediated mitochondrial apoptosis induced by Tea polysaccharide promotes colon cancer cell death. Food Funct. 2021 Sep;: IF ; mouse.
[IF=2.375] Shu-wen Xu. et al. Cigarette smoke extract-induced inflammatory response via inhibition of the TFEB-mediated autophagy in NR8383 cells. EXP LUNG RES. 2023 Jan 13 WB,IF ; Rat.
[IF=2.3] Kawano, Haruna, et al. "Aberrant differentiation of Tsc2-deficient teratomas associated with activation of the mTORC1-TFE3 pathway." Oncology Reports 34.5 (2015): 2251-2258. IHC-P ; Rat.
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| 研究领域 | 细胞生物 免疫学 转录调节因子 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TFEB: 201-300/476 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer. Function: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Subunit: Homodimer and heterodimer; with TFE3 or MITF. Subcellular Location: Cytoplasm. Nucleus. Note=Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus. Post-translational modifications: Sumoylated; does not affect dimerization with MITF. Similarity: Belongs to the MiT/TFE family. Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: P19484 Gene ID: 7942 Database links: Entrez Gene: 7942 Human Entrez Gene: 21425 Mouse Omim: 600744 Human SwissProt: P19484 Human SwissProt: Q9R210 Mouse Unigene: 485360 Human Unigene: 715310 Human Unigene: 2305 Mouse Unigene: 22098 Rat |
| 产品图片 |
Sample:
Muscle (Mouse) Lysate at 40 ug Primary: Anti-TFEB (bs-5137R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD 
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFEB) Polyclonal Antibody, Unconjugated (bs-5137R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
