| 产品编号 | bs-5073R |
| 英文名称 | IDI1 Rabbit pAb |
| 中文名称 | 异戊烯焦磷酸1抗体 |
| 别 名 | IPP1; IPPI1; 4832416K17Rik; IDI1_BOVIN; IDI1; Isopentenyl pyrophosphate isomerase 1 (IPP isomerase 1 | IPPI1); 5.3.3.2; IDI1_HUMAN; IDI1_MOUSE; IDI1_RAT; |
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Specific References (1) | bs-5073R has been referenced in 1 publications.
[IF=5.072] Ni H et al.ErbB4 acts as a suppressor in colitis and its associated carcinoma by negatively regulating cholesterol metabolism.(2018)Carcinogenesis.Nov 17. IP&WB ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 26 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IDI1: 31-130/227 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
IDI1 is a peroxisomally localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). These are substrates for the reactions that ultimately result in the synthesis of cholesterol. There is reduction in IPP isomerase activity in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy. Function: Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). Subunit: Monomer Subcellular Location: Peroxisome. Similarity: Belongs to the IPP isomerase type 1 family. Contains 1 nudix hydrolase domain. SWISS: Q13907 Gene ID: 3422 Database links: Entrez Gene: 3422 Human Entrez Gene: 319554 Mouse Omim: 604055 Human SwissProt: Q13907 Human SwissProt: P58044 Mouse Unigene: 283652 Human Unigene: 644603 Human Unigene: 727514 Human Unigene: 29847 Mouse Unigene: 10780 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IDI1) Polyclonal Antibody, Unconjugated (bs-5073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IDI1) Polyclonal Antibody, Unconjugated (bs-5073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
