| 产品编号 | bs-4061R |
| 英文名称 | GPD2 Rabbit pAb |
| 中文名称 | 线粒体甘油三磷酸脱氢酶2抗体 |
| 别 名 | GDH2; Glycerol 3 phosphate dehydrogenase 2(mitochondrial); Glycerol 3 phosphate dehydrogenase 2; Glycerol 3 phosphate dehydrogenase mitochondrial; Glycerol-3-phosphate dehydrogenase; GPD 2; GPD-2; GPD M; GPD-M; GPDH M; GPDH-M; GPDM; GPDM_HUMAN; mGPDH; mitochondrial; Mitochondrial glycerophosphate dehydrogenase; mtGPD; OTTHUMP00000162788; OTTHUMP00000204452; OTTHUMP00000204453; OTTHUMP00000204458. |
| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 76 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPD2: 165-270/727 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]. Subcellular Location: Mitochondrion. Similarity: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. Contains 2 EF-hand domains. SWISS: P43304 Gene ID: 2820 Database links: Entrez Gene: 2820 Human Entrez Gene: 14571 Mouse Omim: 138430 Human SwissProt: P43304 Human SwissProt: Q64521 Mouse Unigene: 512382 Human Unigene: 3711 Mouse Unigene: 89705 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPD2) Polyclonal Antibody, Unconjugated (bs-4061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
