| 产品编号 | bs-5045R |
| 英文名称 | CPT1B Rabbit pAb |
| 中文名称 | 肉毒碱棕榈酰基转移酶1B抗体 |
| 别 名 | muscle isoform; Carnitine O palmitoyltransferase I mitochondrial muscle isoform; Carnitine O palmitoyltransferase I muscle isoform; Carnitine O-palmitoyltransferase 1, muscle isoform; Carnitine O-palmitoyltransferase I; Carnitine palmitoyltransferase 1A(muscle); Carnitine palmitoyltransferase 1B(muscle); Carnitine palmitoyltransferase 1B; Carnitine palmitoyltransferase I like protein; Carnitine palmitoyltransferase I muscle; Carnitine palmitoyltransferase I-like protein; CPT 1B; CPT I; CPT1 M; CPT1 muscle; CPT1-M; Cpt1b; CPT1B_HUMAN; CPT1M; CPTI; CPTI M; CPTI muscle; CPTI-M; CPTIM; KIAA1670; M CPT1; MCPT1. |
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Specific References (2) | bs-5045R has been referenced in 2 publications.
[IF=4.91] Huebbe, Patricia, et al. "Apolipoprotein E (APOE) genotype regulates body weight and fatty acid utilization–studies in gene targeted replacement mice." Molecular Nutrition & Food Research (2014). WB ; Mouse.
[IF=3.06] Yuan J et al. L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction. Molecules. 2020 Jan 1;25(1). pii: E176. WB ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 85 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CPT1B: 501-600/772 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]. Function: Strong expression in heart and skeletal muscle. No expression in liver and kidney. Subcellular Location: Mitochondrion outer membrane. Similarity: Belongs to the carnitine/choline acetyltransferase family. SWISS: Q92523 Gene ID: 1375 Database links: Entrez Gene: 1375 Human Entrez Gene: 12895 Mouse Omim: 601987 Human SwissProt: Q92523 Human SwissProt: Q924X2 Mouse Unigene: 439777 Human Unigene: 609867 Human Unigene: 112195 Human Unigene: 231465 Mouse Unigene: 43113 Rat |
| 产品图片 |
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-CPT1B antibody (bs-5045R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
