| 产品编号 | bs-2391R |
| 英文名称 | SLC39A7 |
| 中文名称 | 转运蛋白家族39成员7抗体 |
| 别 名 | D6S115E; D6S2244E; H2 KE4; Histidine rich membrane protein Ke4; Histidine-rich membrane protein Ke4; HKE4; HLA class II region expressed gene KE4; KE4; Ke4 gene mouse, human homolog of; Really interesting new gene 5 protein; RING5; S39A7_HUMAN; SLC39A7; Solute carrier family 39 (zinc transporter), member 7; Solute carrier family 39 member 7; solute carrier family 39 zinc transporter member 7; Zinc transporter SLC39A7; ZIP7. |
| 研究领域 | 肿瘤 细胞生物 信号转导 转运蛋白 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Dog,Pig,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC39A7: 201-300/469 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Zinc is an essential cofactor for more than 50 classes of enzymes and is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, plus the control of gene transcription, growth, development, and differentiation. Zinc cannot passively diffuse across cell membranes and requires specific transporters, such as SLC39A7, to enter the cytosol from both the extracellular environment and from intracellular storage compartments. Subcellular Location: Membrane; Multi-pass membrane protein (Probable). Tissue Specificity: Major expression in placenta, lung, kidney and pancreas. Similarity: Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily. SWISS: Q92504 Gene ID: 7922 Database links: Entrez Gene: 7922 Human Entrez Gene: 14977 Mouse Omim: 601416 Human SwissProt: Q92504 Human SwissProt: Q31125 Mouse Unigene: 631995 Human Unigene: 18556 Mouse |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-C39A7 (bs-2391R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD 
Sample:
Lung (Mouse) Lysate at 40 ug Primary: Anti-SLC39A7 (bs-2391R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD 
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC39A7) Polyclonal Antibody, Unconjugated (bs-2391R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC39A7) Polyclonal Antibody, Unconjugated (bs-2391R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
