[IF=19.328] Patricia Altea-Manzano. et al. Reversal of mitochondrial malate dehydrogenase 2 enables anaplerosis via redox rescue in respiration-deficient cells. MOL CELL. 2022 Nov;:  WB ;  Human.  

PubMed:36327975

[IF=6.1] Paul, Subhojit, et al. "STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO 2 gradient." Redox Biology (2017).  WB ;  Rat.  

PubMed:29078168

Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Stomach tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Stomach tissue lysates
Lane 8: Human HL-60 cell lysates
Lane 9: Human MOLT4 cell lysates
Primary: Anti-MDH1 (bs-3996R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MDH1) Polyclonal Antibody, Unconjugated (bs-3996R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-MDH1 antibody (bs-3996)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-DMT1 antibody (bs-3996R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.