| 产品编号 | bs-3975R |
| 英文名称 | AMY2A Rabbit pAb |
| 中文名称 | 胰腺α淀粉酶抗体 |
| 别 名 | AMY2; PA; AMY3; HXA; Amy-X; Amy2-2; Amy2a; mAmy2-1; AMYP_HUMAN; 1,4-alpha-D-glucan glucanohydrolase; 3.2.1.1; AMY2B_HUMAN; AMY2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase; AM2A5_MOUSE; Amy2a5; |
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Specific References (1) | bs-3975R has been referenced in 1 publications.
[IF=4.631] Xiao-Fan Xu. et al. Aspirin Ameliorates Pancreatic Inflammation and Fibrosis by Inhibiting COX-2 Expression in Experimental Chronic Pancreatitis. J INFLAMM RES. 2022 Aug;15:4737-4749 IHC ; Mouse.
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| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58 kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pancreatic Amylase: 421-511/511 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]. Subunit: Monomer. Subcellular Location: Secreted, extracellular space. Similarity: Belongs to the glycosyl hydrolase 13 family. SWISS: P19961 Gene ID: 280 Database links: Entrez Gene: 279 Human Entrez Gene: 280 Human Entrez Gene: 545562 Mouse Omim: 104650 Human Omim: 104660 Human SwissProt: P04746 Human SwissProt: P19961 Human SwissProt: P00688 Mouse Unigene: 484588 Human Unigene: 654437 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Pancreatic Amylase polyclonal antibody, unconjugated (bs-3975R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMY2A) Polyclonal Antibody, Unconjugated (bs-3975R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AMY2A Polyclonal Antibody, Unconjugated (bs-3975R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
