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Rabbit Anti-Glucagon Receptor antibody (bs-3945R)
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说明书: 100ul  200ul
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-3945R
英文名称 Glucagon Receptor
中文名称 胰高血糖素受体抗体
别    名 GCGR; GGR; GL R; GL-R; GLR; GLR_HUMAN; MGC138246; Glucagon receptor precursor.  
Specific References  (1)     |     bs-3945R has been referenced in 1 publications.
[IF=3.73] Rafacho, Alex, et al. "Pancreatic Alpha-Cell Dysfunction Contributes to the Disruption of Glucose Homeostasis and Compensatory Insulin Hypersecretion in Glucocorticoid-Treated Rats." PLOS ONE 9.4 (2014): e93531.  IHC-P ;  Rat.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  细胞生物  免疫学  细胞膜受体  糖尿病  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Guinea Pig, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 51kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Glucagon Receptor:11-110/477 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
The physiologic effects of glucagon are mediated through the glucagon receptor, a member of the superfamily of receptors characterized by a 7-transmembrane domain structure and by their coupling via GTP-binding proteins (G proteins) to adenyl cyclase. The glucagon receptor has been reported to be expressed in liver and adipose, but not in adrenal medulla. ESTs have been isolated from liver/spleen and kidney libraries.

Function:
This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in fetal tissues, including testis, adrenal gland, brain, white blood cells and brain.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

SWISS:
P47871

Gene ID:
2642

Database links:

Entrez Gene: 2642 Human

Entrez Gene: 14527 Mouse

Entrez Gene: 24953 Rat

Omim: 138033 Human

SwissProt: P47871 Human

SwissProt: Q61606 Mouse

SwissProt: P30082 Rat

Unigene: 208 Human

Unigene: 22329 Mouse

Unigene: 11225 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample: Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-Glucagon Receptor (bs-3945R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Glucagon Receptor(bs-3945R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD
Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (bs-3945R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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