| 产品编号 | bs-3937R |
| 英文名称 | ETFB Rabbit pAb |
| 中文名称 | 电子转移黄素蛋白β肽/黄素蛋白抗体 |
| 别 名 | Flavoprotein; beta ETF; FP; beta-ETF; Electron transfer flavoprotein beta polypeptide; Electron transfer flavoprotein beta subunit; Electron transfer flavoprotein subunit beta; Electron transferring flavoprotein beta polypeptide; etfB; ETFB_HUMAN; FP585; MADD. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Cow,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ETFB: 151-255/255 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase. Function: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Subunit: Heterodimer of an alpha and a beta subunit. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas. DISEASE: Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B) [MIM:231680]. GA2B is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Similarity: Belongs to the ETF beta-subunit/fixA family. SWISS: P38117 Gene ID: 2109 Database links: UniProtKB/Swiss-Prot: P38117.3 黄素蛋白是由一条多肽结合1个辅基组成的酶类，不是脂溶性。结合的辅基可以是FAD或FMN,它们是维生素B2的衍生物，每个辅基能够接受和提供两个质子和电子。线粒体中的黄素蛋白主要是电子传递链中NADH脱氢酶和TCA循环中的琥珀酸脱氢酶。 |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- ETFB (bs-3937R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 27 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
